999久久久国产精品,国产情侣一区二区,亚洲AV中文无码乱人伦在线视色,国产欧美一区二区三区在线看,欧美一区精品视频一区二区,色老头在线一区二区三区,又大又粗又硬又爽又黄毛片,国产98在线免费

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > Tetrahymena elliotti Nanney and McCoy
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
Tetrahymena elliotti Nanney and McCoy
Tetrahymena elliotti Nanney and McCoy
規(guī)格:
貨期:
編號:B241816
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Tetrahymena elliotti Nanney and McCoy
商品貨號 B241816
Strain Designations WF 5
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
marsh, South Weare, NH, 1976
Product Format test tube
Type Strain no
Comments
selfer
Medium ATCC® Medium 357: Tetrahymena medium
Growth Conditions
Max Temperature: 27.0°C
Min Temperature: 15.0°C
Duration: axenic
Cryopreservation
RM-9 Media for cryopreservation of Tetrahymena

Proteose Peptone (Difco 0120) ??????????????????????????????????? 5.0 g

Tryptone ???????????????????????????????????????????????????????????????????????????? 5.0 g

K2HPO4??????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? 0.2 g

Glucose ????????????????????????????????????????????????????????????????????????????? 1.0 g

Liver extract??????????????????????????????????????????????????????????????????????? 0.1 g

Glass distilled water???????????????????????????????????????????????????????? 1.0 L

Dissolve components in glass distilled H2O and autoclave.

Dryl?s Salt Solution

0.1 M NaH2PO4 .? 3H20????????????????????????????????????????????????????????????????????????????? 10.0 ml

0.1 M Na2HPO4 . ?7H20????????????????????????????????????????????????????????????????????????????? 10.0 ml

0.1 M Sodium citrate . 2H20 ????????????????????????????????????????? 15.0 ml

0.1 M CaCl2 .? 2H20????????????????????????????????????????????????????????? 15.0 ml

Distilled water?????????????????????????????????????????????????????????????? 950.0 ml

Add the first 3 components to the distilled H2O and mix thoroughly.

Add the CaC12 ?solution and mix thoroughly.

(Adding the solutions in the order indicated will avoid the precipitation of Ca salts.)

1.? Transfer tetrahymena from usual growth medium to RM-9 medium and allow to grow to near peak density.

2.?? Harvest cells from a culture by centrifugation at 300 x g for 2 min.??????????

3.?? Adjust concentration of cells to 2 x 106/ml in fresh

????? medium.

4.?? While cells are centrifuging, prepare a 22% (v/v) sterile

solution of sterile DMSO in fresh medium.

a) Add 2.2 ml of DMSO to an ice cold 20 x 150 mm screw-capped test tube;

b) Place the tube on ice and allow the DMSO to solidify (~5 min) and then add 7.8 ml of ice cold medium;

c) Invert several times to dissolve the DMSO;

d) Allow to warm to room temperature.

5.?? Add a volume of the DMSO solution equal to the cell

????? suspension volume but add in 3 equal aliquots at 2 min

????? intervals. Thus, the final concentration of the preparation

????? will equal 11% (v/v) DMSO and 106 cells /ml.

6.?? Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic

????? screw-capped cryules (special plastic vials for ????? cryopreservation).

7.?? Place the ampules in a controlled rate freezing unit. The

cooling cycle should be initiated no less than 15 min and no longer than 60 min after the addition of the DMSO to the cell preparation. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At? -50°C ampules are plunged into liquid nitrogen.

8.?? Store in the vapor or liquid phase of a nitrogen

????? refrigerator.

9.?? To establish a culture from the frozen state aseptically add 0.5 ml sterile Dryl's Salt Solution to an ampule. Immediately place the ampule in a 35°C water bath, until thawed (2-3 min).? Immerse the ampule just sufficient to cover the frozen material. Do not agitate the ampule.

10. Immediately after thawing, aseptically remove the contents of the ampule and inoculate into 5.0 ml of fresh medium in a 16 x 125 mm screw-capped test tube with a slightly loosened cap. Incubate at 25°C.

CRYOPRESERVATION:

Alternative Thawing Procedure

?1.? Aseptically? add 0.5 ml of sterile modified PYNFH medium (ATCC Medium 1034) containing 8% (w/v) sucrose to the ampule.? Immediately, place in a 35°C water bath, until thawed. Immerse the ampule just sufficient to cover the frozen material. Do not agitate the ampule.

2.?? Immediately after thawing, aseptically remove the contents of the ampule and gently add the material to the edge of a 20 x 100 mm petri plate containing ATCC Medium 919 (non-nutrient agar) and position on a 15 degree slant.  The cell suspension will pool at the edge of the plate.

3.?? Continue to double the volume of the cell suspension at 10

minute intervals by adding ATCC medium 1034) containing 4% sucrose (w/v).  When the volume reaches 16.0 ml place the plate in horizontal position and incubate at 25°C. 

4.?? On the following day, gently remove the cell suspension for the plate and transfer to a T-25 tissue culture flask.  Note the volume of the suspension and add a volume of fresh medium containing 4% sucrose equal to the volume of  the cell suspension.  Incubate the culture at 25°C.

5.?? After culture has been established subculture into fresh

????? normal medium without sucrose. 

Name of Depositor DL Nanney, EM Simon
Chain of Custody
ATCC <<--DL Nanney, EM Simon<<--D. Nyberg
Year of Origin 1976
References

Nanney DL, et al. Comparison of sequence differences in a variable 23S rRNA domain among sets of cryptic species of ciliated protozoa. J. Eukaryot. Microbiol. 45: 91-100, 1998. PubMed: 9495037

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
迁西县| 乌兰浩特市| 锡林浩特市| 通化市| 新竹县| 凌云县| 乌拉特前旗| 吉林省| 闵行区| 武邑县| 宣威市| 金门县| 平罗县| 江城| 寻甸| 祥云县| 南汇区| 河池市| 祁门县| 桃源县| 定安县| 英吉沙县| 阜平县| 公主岭市| 敖汉旗| 灵宝市| 红河县| 龙州县| 卫辉市| 三明市| 泰兴市| 江永县| 三亚市| 鲁甸县| 长宁区| 古蔺县| 梅河口市| 云浮市| 曲松县| 唐河县| 黄冈市|