Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
freeze-dried
Preceptrol®
no
Genotype
DELTA(putPA)101 DELTA(proP)2 DELTA(proU)608
Type Strain
no
Comments
Phenotype(s) verified: spectinomycin resistance.
transformation host deficient in uptake of osmoprotectants, for use with expression vectors pOSEX2 - pOSEX5C (ATCC 87211 - 87216)
Derived from E. coli MC4100.
Medium
ATCC® Medium 1639: LB medium (ATCC medium 1065) with 50 mcg/ml spectinomycin
Growth Conditions
Temperature: 37.0°C
Name of Depositor
E Bremer
Special Collection
NCRR Contract
References
Herbst B, et al. pOSEX: vectors for osmotically controlled and finely tuned gene expression in Escherichia coli. Gene 151: 137-142, 1994. PubMed: 7828862
Haardt M, et al. The osmoprotectant proline betaine is a major substrate for the binding-protein-dependent transport system ProU of Escherichia coli K-12. Mol. Gen. Genet. 246: 783-786, 1995. PubMed: 7898450