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Size (kb): 8.37

Vector: pCM189 (plasmid)

Promoters: Promoter CMV

Marker(s): URA3,ampR

Construct size (kb): 8.37

Features: marker(s): URA3
marker(s): ampR
operator: tetO - seven repeats
other: tTA transcriptional activator
promoter: CMV
promoter: CYC1
replicon: ARS1
replicon: pMB1
MCS: BamHI...PstI
terminator: ADH1
terminator: CYC1
centromere: CEN4

YC-type (centromeric) shuttle vector

expression vector

Restriction digests of the clone give the following sizes (kb): BamHI--8.4; PstI--8.4; BamHI/XhoI--7.7, 0.67.

Yeast centromeric expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors (ATCC 87656 - 87662) differing in the level of expression, mode of replication and selectable marker.

Temperature: 37°C

Component of: ATCC 87663