| 產(chǎn)品名稱 | HeLa |
|---|---|
| 商品貨號(hào) | B164591 |
| Organism | Homo sapiens, human |
| Tissue | cervix |
| Cell Type | epithelial |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 2 [Cells contain human papilloma virus]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | adenocarcinoma |
| Age | 31 years adult |
| Gender | female |
| Ethnicity | Black |
| Applications | These cells are a suitable transfection host. This cell line can be used to screen for Escherichia coli strains with invasive potential. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | Modal number = 82; range = 70 to 164. There is a small telocentric chromosome in 98% of the cells. 100% aneuploidy in 1385 cells examined. Four typical HeLa marker chromosomes have been reported in the literature. HeLa Marker Chromosomes: One copy of Ml, one copy of M2, four-five copies of M3, and two copies of M4 as revealed by G-banding patterns. M1 is a rearranged long arm and centromere of chromosome 1 and the long arm of chromosome 3. M2 is a combination of short arm of chromosome 3 and long arm of chromosome 5. M3 is an isochromosome of the short arm of chromosome 5. M4 consists of the long arm of chromosome 11 and an arm of chromosome 19. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Images |  |
| Clinical Data | 31 years
Black
female |
| HeLa Markers | Y |
| Genes Expressed | Lysophosphatidylcholine (lyso-PC) induces AP-1 activity and c-jun N-terminal kinase activity (JNK1) by a protein kinase C-independent pathway. The cells are positive for keratin by immunoperoxidase staining. |
| Virus Susceptibility | Human adenovirus 3
Encephalomyocarditis virus Human poliovirus 1 Human poliovirus 2 Human poliovirus 3 |
| Comments | The cells are positive for keratin by immunoperoxidase staining. HeLa cells have been reported to contain human papilloma virus 18 (HPV-18) sequences. P53 expression was reported to be low, and normal levels of pRB (retinoblastoma suppressor) were found. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation | Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile | Amelogenin: X CSF1PO: 9,10 D13S317: 12,13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 |
| Isoenzymes | G6PD, A |
| Name of Depositor | WF Scherer |
| Deposited As | Homo sapiens |
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Role of adherence in Interleukin-8 induction in Helicobacter pylori-associated gastritis. Infect. Immun. 65: 3622-3630, 1997. PubMed: 9284128 St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830 Mansky LM. The mutation rate of human immunodeficiency virus type 1 is influenced by the vpr gene. Virology 222: 391-400, 1996. PubMed: 8806523 Dobbelstein M, Shenk T. Protection against apoptosis by the vaccinia virus SPI-2 (B13R) gene product. J. Virol. 70: 6479-6485, 1996. PubMed: 8709286 Churchill MJ, et al. The rev-responsive element negatively regulates human immunodeficiency virus type 1 env mRNA expression in primate cells. J. Virol. 70: 5786-5790, 1996. PubMed: 8709194 Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. 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J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022 Macville M, et al. Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping. Cancer Res. 59: 141-150, 1999. PubMed: 9892199 Dentistry--Preclinical evaluation of biocompatibility of medical devices used in dentistry--Test methods for dental materials. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 7405:1997. |
| Cross References | Nucleotide (GenBank) : X98561 H.sapiens mRNA for dsRNA adenosine deaminase, exon 1C. Nucleotide (GenBank) : X98562 H.sapiens mRNA for dsRNA adenosine deaminase, exon 1D. Nucleotide (GenBank) : U89349 Human papillomavirus type 18 variant, partial sequence. Nucleotide (GenBank) : AJ251709 Homo sapiens partial mRNA for putative protein kinase ERK3. Nucleotide (GenBank) : X84958 H.sapiens mRNA for interferon-induced 17kDa membrane protein. Nucleotide (GenBank) : X98559 H.sapiens mRNA for dsRNA adenosine deaminase, constant region. Nucleotide (GenBank) : AJ251708 Homo sapiens partial mRNA for putative microtubule-binding protein. Nucleotide (GenBank) : NM_003641 Homo sapiens interferon induced transmembrane protein 1 (9-27) (IFITM1), mRNA. Nucleotide (GenBank) : NM_022126 Homo sapiens phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP), mRNA. Nucleotide (GenBank) : AB049629 Homo sapiens lhpp mRNA for phospholysine phosphohistidine inorganic pyrophosphate phosphatase, complete cds. |
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