產(chǎn)品名稱 |
Ray Hot |
商品貨號 |
B162307 |
Organism |
Homo sapiens, human |
Tissue |
skin |
Product Format |
frozen |
Morphology |
fibroblast |
Culture Properties |
adherent |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease |
Ehlers-Danlos syndrome, type III |
Age |
23 years |
Gender |
male |
Clinical Data |
The tissue donor suffered from type III Ehlers-Danlos syndrome, and was the son of Ad Hot (ATCC CRL-1227) who was affected with the same condition. male |
Comments |
The tissue donor suffered from type III Ehlers-Danlos syndrome, and was the son of Ad Hot (ATCC CRL-1227) who was affected with the same condition. |
Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days. |