999久久久国产精品,国产情侣一区二区,亚洲AV中文无码乱人伦在线视色,国产欧美一区二区三区在线看,欧美一区精品视频一区二区,色老头在线一区二区三区,又大又粗又硬又爽又黄毛片,国产98在线免费

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > CRE BAG 2
▍最近瀏覽歷史
▍聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
CRE BAG 2
CRE BAG 2
規(guī)格:
貨期:
編號:B162306
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 CRE BAG 2
商品貨號 B162306
Organism Mus musculus, mouse
Tissue embryo
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease Leukemia
Strain NIH/Swiss
Applications
produces protein beta-galactosidase beta-D-galactosidase
Derivation
The CRE BAG 2 cell line has never produced a replication competent helper virus.
The cells will package vectors derived from murine leukemia viruses.
Genes Expressed
a beta galactosidase transducing vector (BAG),beta-galactosidase(beta-D-galactosidase) [lacZ],beta-galactosidase
Cellular Products
a beta galactosidase transducing vector (BAG)
Comments
The BAG construct has been used to transfect psiCRE cells in ATCC CRL-1858 and to transfect psi2 cells in ATCC CRL-9560.
The CRE BAG 2 cell line has never produced a replication competent helper virus.
This line produces a vector (BAG) that can infect mouse and rat cells and transduce beta-galactosidase activity.
The line is similar to the psi 2 BAG alpha line (see ATCC CRL-9560) but has never been known to produce a replication competent helper virus.
The cells will package vectors derived from murine leukemia viruses.
The vector has been used to infect a variety of neural cell types and to express beta-galactosidase and resistance to G418.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; bovine calf serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin - 0.02% EDTA and allow the flask to sit at room temperature (or 37C) until the cells detach (2 to 3 minutes). Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions
Temperature: 37.0°C
Name of Depositor CL Cepko
References

Price J, et al. Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer. Proc. Natl. Acad. Sci. USA 84: 156-160, 1987. PubMed: 3099292

Turner DL, Cepko CL. A common progenitor for neurons and glia persists in rat retina late in development. Nature 328: 131-136, 1987. PubMed: 3600789

Danos O, Mulligan RC. Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges. Proc. Natl. Acad. Sci. USA 85: 6460-6464, 1988. PubMed: 3413107

Cepko CLVirus propagation and Xgal proceduresIn: Cepko CLNeuromethodsClifton, NJHumana Press1989.

Le Doux JM, et al. Proteoglycans secreted by packaging cell lines inhibit retrovirus infection. J. Virol. 70: 6468-6473, 1996. PubMed: 8709284

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
澳门| 禄劝| 道孚县| 文水县| 五寨县| 洮南市| 卢氏县| 海口市| 扎赉特旗| 綦江县| 成都市| 凯里市| 合山市| 乌兰察布市| 安塞县| 普兰县| 紫金县| 长泰县| 上杭县| 宁明县| 九台市| 晋宁县| 崇礼县| 阜康市| 犍为县| 巴林左旗| 西吉县| 金川县| 吉林省| 博罗县| 微山县| 锡林郭勒盟| 荔浦县| 平昌县| 永兴县| 江津市| 石楼县| 夏河县| 兴和县| 忻州市| 额尔古纳市|