| Application |
The Universal Mycoplasma Detection Kit offers a quick and sensitive PCR-based test to detect mycoplasma contaminants in cell culture. All components required for the PCR reaction are provided and have been optimized for amplification. High specificity is obtained through the utilization of a proprietary mix of buffers, dNTPs and thermostable polymerase, combined with universal primers that are specific to the 16S rRNA coding region in the mycoplasma genome. DNA originating from other sources, such as tissue samples or other bacteria, are not amplified. A touchdown PCR regimen increases sensitivity of the assay, along with enhancing specificity.
The kit detects over 60 species of Mycoplasma, Acholeplasma, Spiroplasma and Ureaplasma including the eight species most likely to afflict cell cultures: M. arginini, M. fermentans, M. hominis, M.hyorhinis, M. orale, M. pirum, M. salivarium, and A. laidlawii. Samples that are positive for mycoplasma are easily recognized by a distinct PCR product ranging in size from 434 to 468 bp on an agarose gel.
| Component |
| Type Strain |
Volume |
| Biosafety Level |
Composition |
| Product Format |
Storage |
| Storage Conditions |
Lysis Buffer |
| 梅經(jīng)理 |
17280875617 |
1438578920 |
| 胡經(jīng)理 |
13345964880 |
2438244627 |
| 周經(jīng)理 |
17757487661 |
1296385441 |
| 于經(jīng)理 |
18067160830 |
2088210172 |
| 沈經(jīng)理 |
19548299266 |
2662369050 |
| 李經(jīng)理 |
13626845108 |
972239479 |
衡东县|
通州市|
徐水县|
吉安市|
贵溪市|
桓台县|
鲁甸县|
营口市|
东光县|
辽中县|
永济市|
滦南县|
雷州市|
南开区|
即墨市|
长乐市|
正安县|
紫金县|
宁明县|
大英县|
会同县|
长子县|
长治县|
电白县|
儋州市|
蒙自县|
遵化市|
明星|
新巴尔虎右旗|
永新县|
响水县|
积石山|
铁岭县|
扶沟县|
新田县|
永吉县|
厦门市|
孝感市|
永安市|
昆山市|
广德县|
|
